TABLE 1.

Comparison of different techniques for cultivation of the poly(3HB)-accumulating thermophile Chelatococcus sp. MW10^a

Fermentation technique	Cultivation scale	OD at 600 nm	CDW (g/liter)	Poly(3HB) content (%, wt/wt)	Poly(3HB) produced (g/liter)	Cell yield (g/g)b	Poly(3HB) yield (g/g)c
Batch culture	250-ml flaskd	18.5 ± 1.4	4.4 ± 0.3	69.0 ± 4.8	3.0 ± 0.4	0.15	0.10
Fed-batch fermentation	2-liter fermentor	22.5 ± 0.2	5.2 ± 0.6	55.5 ± 7.1	2.9 ± 0.7	0.08	0.05
Cyclic batch fermentation	42-liter fermentor
Cycle 1		29.0 ± 0.6	12.7 ± 0.9	55.0 ± 5.7	7.0 ± 1.2	0.25	0.14
Cycle 2		24.6 ± 1.1	11.3 ± 0.4	38.5 ± 6.4	4.3 ± 0.9	0.23	0.09
Cycle 3		19.7 ± 0.5	11.0 ± 0.8	32.5 ± 3.0	3.6 ± 0.6	0.22	0.07
Cyclic fed-batch fermentation	42-liter fermentor
Cycle 1		25.2 ± 0.9	9.1 ± 0.7	40.4 ± 2.8	3.7 ± 0.5	0.23	0.09
Cycle 2		89.0 ± 3.1	43.0 ± 1.4	39.0 ± 8.5	16.8 ± 4.2	0.27	0.11
Cycle 3		174.6 ± 4.7	115.0 ± 4.2	11.8 ± 3.8	13.7 ± 4.9	0.60	0.07

^aFermentation techniques, medium components, feeding solutions, and cultivation conditions were as described in the text. All cultivations were conducted at 50°C. Analyses were done in duplicate; averages and standard deviations are presented.

^bThe substrate conversion factor for cells is given in g CDW/g glucose used.

^cThe substrate conversion factor for poly(3HB) is given in g poly(3HB)/g glucose used.

^dFlask experiments with 30 g glucose/liter (data not shown).