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. 2010 Oct 8;76(23):7843–7853. doi: 10.1128/AEM.01045-10

TABLE 1.

Primers and amplification regimes used in Q-PCR

Group Primer 1 Primer 2 Annealing temp (°C)/time (s) Reading temp (°C)/time (s) Reference(s)
Total bacteria Ba519F Ba907R 52/30 25, 26
amoA AmoA-1F AmoA-2R-TC 52 /60 81/15 31
Chitinase group A GA1F GA1R 63/30 46