FIG. 2.

Inc-P1 replicon constructs bearing the pemI/pemK plasmid addiction system. Maps of plasmids derived from pXF-RIV11 (A) or pVEIS01 (B) are depicted. ORFs are denoted by black arrows, and various origins of replication are indicated as gray arcs. The larger constructs (pXF19-PEMIK and pVEL4-PEMIK) differ from the corresponding smaller constructs (pXF20-PEMIK and pVES3-PEMIK) by containing the complete resolvase ORF (res) of the respective IncP-1 plasmid donor located upstream of the cognate trfA ORF. Locations of multiple cloning sites (mcs1 and mcs2) are indicated. Panels C and D show NotI digests (excising pemI/pemK) of plasmids used to transform X. fastidiosa (lanes O) or rescued from X. fastidiosa by transformation of E. coli (lanes R).