TABLE 3.
Characteristics of constructed XYL1 librariesa
| Mutagenesis range |
E. coli sublibrary |
S. cerevisiae library |
||
|---|---|---|---|---|
| Size (CFU) | % Completion | Size (CFU) | % Completion | |
| +631-+870 | 1.3 × 106 | DNA (1), 100; DNA (2), 69 | 6.3 × 105 | DNA (1), 100; DNA (2), 39; protein (1), 55 |
a
The completion levels of the E. coli sublibrary and the S. cerevisiae library refer to the predicted contents of discrete mutations of a certain kind divided by the number of possible variants. DNA (1) refers to the fraction of all possible single-nucleotide mutations, DNA (2) refers to the fraction of all possible two-nucleotide mutations, and protein (1) refers to the fraction of all possible single-amino-acid mutations. The numbers were calculated using the PEDEL online tool (31).