FIG. 5.

Glut can specifically induce muscle contractions in the coculture, which can be stopped by curare. (A) Coimmunostaining of β-III Tubulin (blue), choline acetyl transferase (ChAT) (red), and GlutR (green) in a day 31 culture. As indicated, while all the neurons are positive for β-III Tubulin, those that are positive for ChAT also show high level expression of GlutRs. (B) Specific muscle contractions were induced in the MN-eSKM cocultures (Supplemental Videos S1–S4; available online at www.liebertonline.com/ten). GlutRs, glutamate receptors. Color images available online at www.liebertonline.com/ten.