Table 1.
Composition of the Enriched Coculture Medium
| Component | Full name | Concentration | Company | Catalog number |
|---|---|---|---|---|
| Neurobasal/neurobasal A | Invitrogen | 10888/21103 | ||
| B27 (50×) | 1× | Invitrogen | 17504-044 | |
| Glutamax (100×) | 1× | Invitrogen | 35050 | |
| GDNF | Glial-derived neurotrophic factor | 10 ng/mL | Cell Sciences | CRG400B |
| BDNF | Brain-derived neurotrophic factor | 20 ng/mL | Cell Sciences | CRB600B |
| Shh | Sonic hedgehog, N-terminal peptide | 50 ng/mL | R&D | 1845-SH-025 |
| RA | Retinoic acid | 0.1 μM | Sigma | R2625 |
| IGF-1 | Insulin-like growth factor-1 | 10 ng/mL | PeproTech | 100-11 |
| cAMP | Adenosine 3′,5′-cyclic monophosphate | 1 μM | Sigma | A9501 |
| CNTF | Ciliary neurotrophic factor | 5 ng/mL | Cell Sciences | CRC400A |
| NT-3 | Neurotrophin-3 | 20 ng/mL | Cell Sciences | CRN500B |
| NT-4 | Neurotrophin-4 | 20 ng/mL | Cell Sciences | CRN501B |
| Vitronectin | 100 ng/mL | Sigma | V8379 | |
| Laminin | Mouse laminin | 4 μg/mL | Invitrogen | 23017-015 |
| G5 (100×) | 1× | Invitrogen | 17503-012 |