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. Author manuscript; available in PMC: 2011 Nov 2.
Published in final edited form as: Biochemistry. 2010 Nov 2;49(43):9207–9216. doi: 10.1021/bi100819v

Figure 2.

Figure 2

(A) Representative traces of whole-cell current measured from a single HEK-293 cell expressing the wild-type hGluK2. Glutamate was applied at time zero, and the concentrations of glutamate were, from bottom up, 2, 0.5, 0.2, 0.1 and 0.05 mM, respectively. (B) Dependence of desensitization rate constant on glutamate concentration for the wild-type hGluK2 (black circle) and the M867I mutant (red circle). Each data is an average of at least three data points from three cells. A total of 39 and 29 cells were measured for the wild-type and mutant GluK2s, respectively. (C) Dependence of desensitization rate constant on glutamate concentration for the wild-type rGluK2. A total of 52 cells were measured, and each data as shown is an average of at least three data points from three cells. (D) Representative whole-cell current traces for the wild-type rGluK2 (left panel), wild-type hGluK2 (middle panel) and M867I mutant hGluK2 (right panel). All traces were collected at 2 mM glutamate. The desensitization rate constant is, from left to right, 234, 212 and 118 s−1, respectively.