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. 2010 Oct 28;10:314. doi: 10.1186/1471-2334-10-314

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Copyright ©2010 Woo et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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SLF-derived MCP-1/CCL2 is associated with inner ear inflammation secondary to NTHI-induced OM in vivo. Murine temporal bones were harvested at 7 days after transtympanic inoculation of saline (A) and live NTHI (B). Immunolabeling shows MCP-1/CCL2 is highly expressed in the spiral ligaments (white arrows) and spiral limbus as well as the serous substances (black arrow) of the NTHI-inoculated mice. In contrast, it is noted that the cochlea of the saline-inoculated group weakly expresses MCP-1/CCL2 except the stria vascularis (arrowheads). Original magnification: ×50.