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. 2010 Nov 1;8:131. doi: 10.1186/1477-7827-8-131

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Copyright ©2010 Duffy et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Detection of EP mRNA in monkey oocytes. Products of the expected size were obtained by RT-PCR for EP1 (203 base pair (bp)), EP2 (253 bp), EP3 (251 bp), EP4 (228 bp), and β-actin (270 bp). Amplified product was not obtained when water was substituted for reverse transcribed monkey RNA. Position of DNA base pair standards shown on left (bp). Below gel, the number of oocyte preparations with detectable EP receptor mRNA is shown out of the total of 6 preparations tested; β-actin mRNA was detected in all 6 preparations tested.