Figure 7. Measure of Ka/Ks ratios and Tajima's D on the coding region of pin-c (NCU03494) show evidence for balancing selection between allele classes.

Ka/Ks ratios (ω, ratio of nonsynonymous (Ka) to synonymous (Ks) nucleotide substitution rates; red) were calculated for each codon for all pin-c alleles and significant positive selection between positions 77–746 (p = 0.001) by likelihood ratio test was detected. These positions include the HET domain (codons 335–533) of pin-c (NCU03494)(large bar on x-axis). Tajima's D calculated for each codon for all pin-c alleles shows significant balancing selection (P<0.1) at positions 8–78 and 190–390 (smaller bars on x-axis; see E and F). Evaluation of Ka/Ks (red) and Tajima's D (blue) within a pin-c allele class shows variable selection: B) A region from codon 60 to 150 shows evidence of balancing selection in pin-c1 alleles (shown as a bar on the x axis) C) pin-c2 D) pin-c3. pin-c3 alleles show an extremely high ω with three very polymorphic regions (codons 448–491, 569–639 and 738–781). E and F) Amino acid alignments of putative allelic specificity regions of pin-c (the locations of these regions are indicated by bars on the x-axis of panel A).