Figure 1. Tail-truncated CXCR4 is able to bind CXCL12.

(A) Snake-plot of mCXCR4 highlights two signaling modules, the DRY motif in green and the serine and threonine residues in the CT tail of CXCR4 in orange. The arrowhead indicates the site of truncation in ΔT mice. (B) Histograms show the expression of CXCR4 on WT (shaded histogram) or ΔT FL cells (red line) isolated from E18.5 embryos. The histogram with black line represents isotype control. (C) Percentages of ¹²⁵I-CXCL12 bound to HEK cells expressing WT CXCR4 (black curve) or ΔT (red curve) in the presence of increasing concentrations of unlabelled CXCL12 (n = 3, mean ± SD).