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. 2010 Nov 19;5(11):e15397. doi: 10.1371/journal.pone.0015397

Figure 4. ΔT largely restores the defective BM retention and cell adhesion of CXCR4-null B-lineage precursors.

Figure 4

(A) Peripheral blood cells and splenocytes were isolated from 6 wk old WT, CD19-Cre+ CXCR4f/f and CD19-Cre+ CXCR4f/ΔT mice, and stained with anti-CD19, B220 and IgM. CD19+-gated cells can be further distinguished as B-cell precursors (B220loIgM−) and mature B cell (B220hiIgM+) populations. Bar graphs show percentages of B-cell precursors within the total CD19+ B-cell population detected in the periphery. Results were obtained from 5–8 mice of each group (*, P<0.001; **, P<0.000005, calculated by the two-tailed Student's t test). (B) CXCL12-stimulated WT, CXCR4-null and ΔT pro-B cells were analyzed for their ability to adhere to VCAM-1. Data are displayed as mean ± SD from three independent experiments (*, P<0.001; **, P<0.0005, calculated by the two-tailed Student's t test).