Effect of ethanol (40 mM) on the pacemaker activity of Purkinje neurons in parasagittal cerebellar vermis slices. (A), representative traces showing action currents recorded in loose-seal cell-attached configuration in Purkinje neurons from 30 day-old Sprague-Dawley male rats before (baseline), during (ethanol) and after (washout) application of 40 mM ethanol. (B), time course of the normalized firing frequency recorded from these Purkinje neurons (n = 8). (C), Same as in A but for 58-60 day-old rats. (D), Same as in B but for 58-60 day-old rats (n = 8). All values are expressed as mean ± SEM.