Table 2. Excision frequencies of the ISDra2-113 derivative in a Deinococcus radiodurans tester straina expressing TnpADra2 variants.
Resident plasmid | TnpADra2 expressed in trans | Excision frequency |
---|---|---|
No | No | <10−8 |
pGY11559 | No | <10−8 |
pGY13203 | TnpA wt | 3.2 (±0.6) × 10−3 |
pGY13521 | TnpAR14A | 5.3 (±1.6) × 10−5 |
pGY13522 | TnpAS122G/E123G | 1.7 (±0.3) × 10−3 |
aGY13115 genotype: ΔDR1652ΔDR1651ΩtetAΩISDra2-113 (CamR) (Pasternak et al, 2010). |