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. 2010 Sep 28;29(22):3826–3839. doi: 10.1038/emboj.2010.242

Figure 2.

Figure 2

UvrD monomers can initiate translocation from a 5′-ss/dsDNA junction. (A, B) N-step sequential model simulation of translocation monitoring arrival of translocase at 5′-end of ssDNA assuming different initial binding distributions, random and 3′-end, respectively. (C) Time courses of UvrD monomer translocation on fluorescein-labelled ssDNA (5′-F-dT54) and 5′-ss/dsDNA (5′-F-dT54-DP18). (D) Time courses of UvrD translocation on 5′-F-dTL-DP18 as a function of 5′-tail length (L=16, 25, 36, 44, 79, 84, 97 and 114). (E) Simulated time courses monitoring UvrD arrival at the 5′-end of 5′-F-dT79-DP18 varying the probability of UvrD initially bound at junction (P(UvrD,junction)). (F) Time-to-peak analysis, see text, of the time courses in (D).