UvrD monomers at the ss/dsDNA junction can loop out ssDNA over a short distance during translocation. (
A) Cartoon depicting FRET experiment. (
B) Control experiments showing anti-correlated signal changes in Cy3 (
) and Cy5 (
) signals as the length of the FRET substrate 5′-tail increases along with the signal from donor only substrates (
). Lines drawn to show trends and error bars represent s.d. of the average of three separate experiments. (
C) Time courses of UvrD monomer translocation on FRET (lines) and donor 5′-dT
L-DP
18 (scatter) substrates (
L=25, 35, 44 and 65). (
D) Time courses of UvrD monomer translocation on FRET 5′-dT
L-DP
18 substrates (
L=25, 44 and 65) obtained by exciting Cy5.
