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MEKK1 needs Han11 for coupling to HIPK2/DYRK1. Cells were transfected to express HA-tagged MEKK1 together with Flag-tagged HIPK2 (A), DYRK1a (B) or DYRK1b (C) either alone or together with Han11. Equal amounts of proteins were immunoprecipitated with anti-HA or adequate control antibodies and tested for the occurrence of the (co)immunoprecipitated proteins and the input material as indicated. (D) Schematic summary of the protein/protein interaction experiments.
