Table 2.
Definitions for homozygous and hemizygous p16/CDKN2A deletion determined by fluorescence in situ hybridisation (FISH)
| p16/CDKN2A deletion pattern | FISH signal pattern | Cut-off value for % nuclei with FISH signal pattern | |
| p16/CDKN2A (SpO) | CEP-9 (SpG) | ||
| Homozygous | No p16/CDKN2A signal 0SpO |
At least one CEP-9 signal 1-2SpG |
>10% |
| Hemizygous | One p16/CDKN2A signal 1SpO |
Two CEP-9 signals 2SpG |
>15% |
| Hemizygous (monosomy) | One p16/CDKN2A signal 1SpO |
One CEP-9 signal 1SpG |
>44% |
SpO, Spectrum Orange; SpG, Spectrum Green.