Table 1.
Immunohistochemical staining protocol
| Antibody | Source | Concentration | Pre-treatment |
|---|---|---|---|
| CK-5 | Zytomed Systems, Berlin, GE | 1:75 | Boiling |
| CK-6 | BioLogo, Kronshagen, GE | 1:10 | Enzymatic |
| CK-7 | Zytomed Systems, Berlin, GE | 1:100 | Enzymatic |
| CK-8 | Zytomed Systems, Berlin, GE | 1:50 | Enzymatic |
| CK-10 | Zytomed Systems, Berlin, GE | 1:100 | None |
| CK-13 | Zytomed Systems, Berlin, GE | 1:30 | Boiling |
| CK-15 | Acris, Herford, GE | 1:50 | Enzymatic |
| CK-18 | Dako, Hamburg, GE | 1:100 | Enzymatic |
| CK-19 | Dako, Hamburg, GE | 1:50 | Enzymatic |
| CK-20 | BioLogo, Kronshagen, GE | 1:200 | Boiling |
| AE1/AE3 | Zytomed Systems, Berlin, GE | 1:140 | Boiling |
| Galectin-3 | Santa Cruz Biotech., Santa Cruz, USA | 1:50 | Enzymatic |
Boiling procedure 10 min. in citrate buffer, pH 6.0, in a pressure cooker
Enzymatic 0.1% pronase E (Merck, Darmstadt, GE, K38823533845) 10 min., 37°C