Figure 6.

Expression levels of LPA₁ receptor in the dorsal root, spinal nerve and sciatic nerve fibers. (a) The mRNA expression levels of LPA₁ receptors in the dorsal root ganglion (DRG), dorsal root (DR), spinal nerve (SPN) or sciatic nerve (SCN) fibers in vivo, assessed by RT-PCR. β-actin was used as an internal control. Photographs show the representative image of RT-PCR. Data were obtained from three independent experiments. (b) Protein expression levels of LPA₁ receptors in the dorsal root (DR), spinal nerve (SPN) or sciatic nerve (SCN) fibers in vivo, using western blot analysis. β-tubulin (β-Tub) was used as an internal control. Photographs show the representative image of western blot. Data were obtained from three independent experiments.