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. 2010 Nov 10;11:144. doi: 10.1186/1471-2202-11-144

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Copyright ©2010 Chaves et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Alpha-synuclein isoforms. Western blot illustrating the isoforms of alpha-synuclein present in the substantia nigra of adult and newborn Wistar and Lewis rats using antibody from Abcam (A) and Santa Cruz (B), confirming the absence of the 14 kDa isoform of alpha-synuclein in neonate Lewis rats. The antibody from Abcam showed 3 isoforms of alpha-synuclein (90 kDa, 40 kDa and 14 kDa). Antibodies specificity was demonstrated by adding blocking peptide to the solution containing antibodies, which prevented the label of bands corresponding specifically to alpha-synuclein (C).