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Quantification of alpha-synuclein aggregation after rotenone exposure. Expression of alpha-synuclein isoforms of 90 kDa and 40 kDa in cell cultures of substantia nigra following incubation with DMSO or 0.5 nM of rotenone during 48 h. Normalization was performed by beta-actin (43 kDa) signal. Data are shown as percent of control (DMSO) ± S.D. * p < 0.05 as compared to control (DMSO), according to Student's t-test. The experiment was repeated twice, which were run in triplicates.
