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. 2010 Nov 8;10:614. doi: 10.1186/1471-2407-10-614

Figure 1.

Figure 1

Transfection of Daoy medulloblastoma cell line and U87MG astrocytoma cell line with GLI1 siRNA. Figure 1A: Daoy cell line is transfected with Stealth GLI1 siRNA (Invitrogen) with 100 pmol concentration, using Lipofectamine™ 2000 as a transfecting agent and fluorescein-labeled oligo (BLOCK-iT™ Fluorescent oligo) for identification of trasfection efficency. Figure 1B: Daoy cell line transfected with universal negative (U neg) siRNA. Figure 1C: Untransfected Daoy cell line. Figure 1D: U87MG cell line is transfected with Stealth GLI1 siRNA (Invitogen) with 100 pmol concentration, using Lipofectamine™ 2000 as a transfecting agent and fluorescein-labeled oligo (BLOCK-iT™ Fluorescent oligo) for identification of trasfection efficency. Figure 1E: U87MG cell line transfected with universal negative (U neg) siRNA. Figure 1F: Untransfected U87MG cell line.