(A) Daxx activates JNK in transient transfection. Flag-tagged JNK1 (Flag-JNK) and the indicated plasmids (1 μg each) were cotransfected into 293 cells. Top: phosphorylation of GST-cJun. Bottom: expression of Flag-JNK. The data shown are representative of four independent assays.
(B) Stable expression of Daxx constitutively activates JNK. Top: phosphorylation of GST-cJun. Bottom: expression of endogenous JNK. The data shown are representative of three independent assays.
(C) Daxx activates a JNK-dependent reporter gene. The data shown are the average and SD of three independent experiments in duplicate.