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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Anal Chem. 2010 Oct 1;82(19):8113–8120. doi: 10.1021/ac1011415

Figure 1.

Figure 1

Diagram of HPLC-LIF-MS setup. The fused silica (FS) capillary (365 μm O.D., 50 μm I.D.) connecting the HPLC column and the ESI source was 30 cm long. The detection window was formed by burning an approximately 1 cm window in the polyimide coating. The interface between the fused-silica capillary and electrospray source was accomplished using a Teflon sleeve (Upchurch, F-242X) and a PEEK finger-tight fitting (Upchurch, F-300X). The ESI-MS instrument was a Bruker MicrOTOFQ mass spectrometer. The fluorescence cell (B) was obtained from SpectrAlliance and guides incident laser light (473nm) and fluorescent light via fiber optics (O.F., 1 m in length). Optical fibers, internal to the cell, guide incident light and fluorescent light at a 90° angle. PMT is a photomultiplier tube biased at 800 V; fluorescence was selected with a 580BP45 optical filter in front of the PMT; the data acquisition rate was 10 Hz.