Fig. 1.

Verification of the experimental set-up for capturing extracellular traces. (A) Typical recording from the IML of a spinal cord slice. Left: extracellular recording. Right: power spectrum of recorded activity with a range of frequencies between 5 and 30 Hz. (B) Placing the electrode in the bathing solution shows the level of background noise. Note the difference in scale. (C) Comparison of extracellular activity recorded in the same slice from three different regions: lamina IX of ventral horn, Lamina II of dorsal horn and the IML. Each trace is accompanied by the autocorrelogram (left) and power spectrum (right). Oscillations were present only in the IML. AC, autocorrelation coefficient. (D) Rhodamine dye at the recording site shows correct positioning in the IML. Images show the same area of spinal cord revealing rhodamine fluorescence (right), or structure of the cord (left). Arrow indicates the dorsolateral border of the IML.