Fig. 5.

5-HT induces oscillatory activity in the IML. (A) Low-pass filtered (<35 Hz) extracellular voltage recording from the IML of a single spinal cord slice before (left) and after (right) application of 10 μM 5-HT. Rhythmic oscillations appear during 5-HT application. (B) Power spectral analysis of a 1 min segment of control (left) and 5-HT (right) activity showing a prominent peak at 9.4 Hz. (C) Autocorrelograms showing that the activity is self-similar (rhythmic) only after 5-HT is applied. (D) Surface plot of power spectra taken at consecutive time points from a different slice, showing the development and decline of an 11.9 Hz oscillation over time. (E) Bar chart of the mean data showing that the mean oscillation frequency was not affected by 5-HT (n=5). Error bars=SEM. (F) Box plot of oscillation power (as for Fig. 2). 10 μM 5-HT significantly increased the power of the oscillation. * P<0.05, Wilcoxon matched pairs signed rank test.