Figure 3.

Immunohistochemistry of eNOS in animals of the control (Con), ovariectomy (Ovx), and ovariectomy followed by 17β-estradiol treatment (Ovx+Est) groups. Immunoreactivity of endothelial nitric oxide synthase (eNOS) was detected in the capillaries and venules. The eNOS protein expression was significantly reduced after ovariectomy, but was restored to the control value after 17β-estradiol treatment. The right panels denote the means±standard deviation of 10 experiments for each condition determined by densitometry relative to GAPDH. ^*p<0.05 vs. control. ^**p<0.05 vs. Ovx. Con=control; Ovx=ovariectomy; Ovx+Est=ovariectomy plus 17β-estradiol treatment. Horizontal scale bar at the bottom of each figure indicates the magnification power.