. Author manuscript; available in PMC: 2010 Nov 22.

Published in final edited form as: Proteomics. 2008 Sep;8(18):3848–3861. doi: 10.1002/pmic.200700969

Table 1. List of all identified proteins.

The table lists the accession number, the number of individual peptides matched, the percentage coverage of the identified protein sequence and the Mascot score of the protein. When the accession number matched to an unknown protein in the database, the identity of the protein was determined by searching, using the BLAST algorithm, the amino acid sequence of the identified peptides in the NCBI databases. The proteins were classified according whether they were previously identified as associated with endocrine secretory granules (ESG), or according to their most often reported localization (PubMed searches) into the following categories: SK: Cytoskeleton, NU: Nucleus, ER: Endoplasmic Reticulum, GO: Golgi Apparatus, EN: Endosome, MI: Mitochondria PR: Proteasome, CY: Cytosol, RI: Ribosome, PM: Plasma Membrane, RI: Ribosome, Variable (proteins having multiple major localization sites or for which not enough information is available). For ESG proteins, a study in which association with endocrine secretory granules was observed, is referenced. When present in ESG as shown by proteomics, it is labeled with a P and referenced.

Name	Accession	Peptides	Cov%	Score	Localization	Study
14-3-3 protein epsilon	gi\|74189349	8	35	408	ESG	[45]
14-3-3 protein gamma	gi\|3065929	7	25	351	ESG	P [23]
14-3-3 zeta	gi\|1841387	11	44	576	ESG	P [23]
78 kDa glucose-regulated protein	gi\|1304157	9	16	480	ER
Acrogranin	gi\|50852	4	11	230	Variable
Actin, gamma	gi\|74213524	21	64	1107	ESG	P [23]
Actinin, alpha 1	gi\|32766260	2	2	129	ESG	[46]
Adenomatous polyposis coli protein	gi\|12643510	3	1	50	SK
Alpha-NAC	gi\|1666692	4	2	252	NU
Asparagine synthetase B	gi\|26345208	3	6	94	Variable
ATP synthase, beta subunit	gi\|23272966	4	10	136	ESG	P [23]
ATPase, Na/K transporting, alpha 1 polypeptide	gi\|21595127	23	27	1115	PM
ATPase, Na/K transporting, beta subunit	gi\|54130	4	18	156	PM
Calmodulin	gi\|74143933	8	47	573	ESG	[47]
Calreticulin	gi\|26344403	3	9	107	ER
CAP, adenylate cyclase-associated protein 1	gi\|26343401	2	7	117	SK
Carnitine deficiency-associated gene 3B	gi\|28461294	5	38	203	Variable
CAST1/ERC2	gi\|38231910	3	5	54	ESG	[48]
Catenin (Ctnnd1) p120	gi\|26006157	3	4	152	PM
Cellular nucleic acid binding protein	gi\|50471	2	16	85	NU
Chaperonin containing TCP-1 delta subunit	gi\|460317	2	4	83	ESG	[49]
Chaperonin containing TCP-1 theta subunit	gi\|5295992	4	8	91	ESG	[49]
Chaperonin containing TCP-1, beta subunit	gi\|468546	3	8	97	ESG	[49]
Chaperonin subunit 5	gi\|37359776	4	9	243	Variable
Chaperonin subunit 6a	gi\|17391080	2	9	79	Variable
Charged multivesicular body protein (CHMP4b)	gi\|74192743	4	22	117	EN
Chromogranin A	gi\|20071660	26	38	1440	ESG	P [23]
Chromogranin B	gi\|50409	18	29	887	ESG	P [22]
Clathrin, heavy polypeptide	gi\|33438248	6	4	233	ESG	P [22]
Coatomer protein complex, gamma subunit	gi\|19354315	2	4	58	ER, GO
Cofilin 1	gi\|12861068	2	9	64	SK
Complexin 2	gi\|62740137	2	20	84	ESG	[39]
Cp27	gi\|3115274	2	10	56	PM
Cu/Zn superoxide dismutase	gi\|226471	8	50	489	ESG	P [23]
Cyclophilin A (Peptidyl-prolyl cis-trans isomerase A)	gi\|74146841	9	66	407	ESG	P [23]
Cysteine-rich protein 1	gi\|74212172	4	71	127	SK
DEAD box polypeptide 3	gi\|18204785	3	5	147	NU
Desmoplakin	gi\|82950147	5	2	111	SK
Diazepam binding inhibitor	gi\|67511482	2	25	110	ESG	[50]
Dynein, heavy chain 1	gi\|13384736	4	1	70	ESG	[51]
Dynein, heavy chain 8	gi\|14335452	4	1	53	ESG	[51]
Dynein, light chain roadblock-type 1	gi\|12842877	2	10	56	SK
EEF2	gi\|26324898	5	7	165	NU
EIF4a1	gi\|50820	4	13	146	NU
EIF4h	gi\|39104480	4	23	121	NU
Elongation factor Tu	gi\|26345590	17	40	819	NU
Enolase 1, alpha	gi\|53734652	16	50	1030	Variable
Fatty acid synthase	gi\|74142919	3	2	129	PM
Fatty acid-binding protein, epidermal	gi\|74191310	6	40	246	ESG	[52]
Fructose-bisphosphate aldolase A	gi\|7548322	17	53	687	ESG	P [23]
Gag-pro-pol polyprotein	gi\|559059	8	17	245	Variable
Gasdermin 1	gi\|26325036	3	9	102	Variable
Glucose phosphate isomerase	gi\|56554102	7	18	309	ESG [53]	[53]
Glyceraldehyde-3-phosphate dehydrogenase	gi\|62201487	14	49	725	ESG	P [23]
Glycyl-tRNA synthetase	gi\|18256043	2	2	55	Variable
GP42/Basigin protein	gi\|631683	5	21	212	PM
Guanine nucleotide binding protein, alpha subunit, inhibitory	gi\|309255	2	4	83	ESG	[54]
Guanine nucleotide binding protein, alpha subunit, stimulating	gi\|47271396	7	8	232	ESG	P [22]
Guanine nucleotide binding protein, beta 2 subunit	gi\|984551	7	23	232	ESG	P [22],[23]
Heat shock protein hsp70	gi\|74220592	25	43	1181	ESG	[55]
Heat-shock protein hsp84/90	gi\|74147026	29	39	1330	Variable
Heterogeneous nuclear ribonucleoprotein K	gi\|12847547	2	4	52	Variable
HN1	gi\|74220270	2	27	125	Variable
HN1-like protein	gi\|74213941	4	40	192	Variable
Importin beta 1	gi\|2829480	3	5	144	Variable
Integrin alpha 6	gi\|110577	3	19	58	PM
IQ motif containing GTPase activating protein 1	gi\|27370648	3	4	70	SK
Lactate dehydrogenase 1	gi\|74198692	13	45	636	Variable
L-type amino acid transporter 1	gi\|6906727	2	5	145	PM
M2-type pyruvate kinase	gi\|74221210	23	54	1389	Variable
Macrophage migration inhibitory factor	gi\|5822094	3	23	69	ESG	[41]
MARCKS-like 1	gi\|13879553	2	14	65	PM
Microtubule-actin crosslinking factor 1	gi\|94372354	16	2	162	SK
Monocarboxylate transporter 4	gi\|7688756	2	5	54	PM
Myosin, heavy polypeptide	gi\|20137006	19	12	705	ESG	P [22]
Myosin, light polypeptide	gi\|16924329	6	52	345	ESG	[56]
Myosin, regulatory light chain 2	gi\|12851268	3	20	121	ESG	[56]
Myosin-I, beta	gi\|1924961	2	3	88	SK
Myristoylated alanine rich protein kinase C substrate (MARCKS)	gi\|6678768	2	11	71	PM
Nebulin	gi\|94366489	14	1	126	SK
Neural cell adhesion molecule 1 (NCAM)	gi\|817984	10	41	480	ESG	[57]
Nucleolin	gi\|13529464	6	7	168	NU
Nucleosome assembly protein 1-like 1	gi\|50722	3	11	109	NU
Oxidation resistance protein 1	gi\|58047714	3	4	51	PM
P23	gi\|12843224	2	16	122	Variable
PDGFA associated protein	gi\|74151229	5	32	153	Variable
Peptidylglycine alpha-amidating monooxygenase	gi\|7305367	4	3	193	ESG	P [23]
Peroxiredoxin 1	gi\|12846314	3	21	67	Variable
Phosphatidylethanolamine binding protein	gi\|1517864	4	31	113	ESG	[40]
Phosphofructokinase-1 C isozyme	gi\|74224916	4	10	82	PM
Phosphoglycerate kinase 1	gi\|129903	7	23	438	Variable
Phosphoglycerate mutase 1	gi\|12844989	3	13	95	Variable
Plakoglobin	gi\|423532	2	4	102	SK
Profilin 1	gi\|26389590	2	19	64	SK
Prolyl 4-hydroxylase	gi\|54777	8	17	163	ER
Proopiomelanocortin (POMC)	gi\|74227361	9	45	517	ESG	[58]
Proprotein convertase PC1/3	gi\|7305371	2	2	79	ESG	P [22]
Proteasome, 26S non-ATPase subunit 2	gi\|12861131	2	28	96	PR
Proteasome, delta subunit	gi\|74191020	2	17	66	PR
Protein disulfide isomerase associated 3	gi\|6679687	8	17	247	ESG	P [23]
Rab10	gi\|10435058	2	11	112	ESG	P [23]
Rab14	gi\|10435483	2	8	70	ESG	P [22],[23]
Rab1A	gi\|74147521	2	13	109	ESG	P [23]
Radixin	gi\|74186081	6	8	106	SK
Rap1a	gi\|53236951	3	15	67	ESG	P [23] [59]
Regulator G-protein signaling 9	gi\|2739458	4	6	53	PM
Ribosomal protein L10A	gi\|6755350	3	13	86	RI
Ribosomal protein L10E	gi\|71051393	3	11	108	RI
Ribosomal protein L15	gi\|12846287	3	15	82	RI
Ribosomal protein L17	gi\|12832997	3	16	88	RI
Ribosomal protein L18	gi\|12840700	3	19	132	RI
Ribosomal protein L19	gi\|6677773	2	9	66	RI
Ribosomal protein L21E	gi\|94368373	4	24	98	RI
Ribosomal protein L23	gi\|12849613	2	25	160	RI
Ribosomal protein L26	gi\|74179650	3	16	94	RI
Ribosomal protein L3	gi\|74198856	3	6	77	RI
Ribosomal protein L6	gi\|695638	6	20	133	RI
Ribosomal protein L8	gi\|74203516	3	10	77	RI
Ribosomal protein P2	gi\|74140891	5	70	290	RI
Ribosomal protein S10	gi\|74198792	2	10	72	RI
Ribosomal protein S16	gi\|7305445	3	20	54	RI
Ribosomal protein S18	gi\|198578	2	9	85	RI
Ribosomal protein S2	gi\|12835827	2	8	70	RI
Ribosomal protein S20	gi\|74181462	3	22	73	RI
Ribosomal protein S23	gi\|12846275	2	13	68	RI
ribosomal protein S28	gi\|12833257	3	46	140	RI
Ribosomal protein S3	gi\|12847921	2	11	57	RI
Ribosomal protein S4	gi\|12846200	4	15	128	RI
Ribosomal protein S8	gi\|26353710	3	17	138	RI
Secretogranin II	gi\|54096	2	2	79	ESG	P [23]
Secretogranin III	gi\|74211039	3	11	147	ESG	P [22],[23]
Serpin1	gi\|12836024	3	9	81	ESG	[60]
Seryl-aminoacyl-tRNA synthetase 1	gi\|26349967	3	5	89	MI
Similar to PEST-containing nuclear protein (PCNP)	gi\|74141568	2	21	94	NU
Similar to tumor protein, translationally-controlled 1 (TCTP)	gi\|74181622	2	5	55	PM
Similar to ubiquitin C	gi\|94375393	3	23	93	ESG	P [22]
Solute carrier family 3 (CD98)	gi\|26354873	12	28	528	PM
Spectrin, beta 2	gi\|7106421	2	1	54	ESG	[61]
Stathmin	gi\|12832714	13	63	463	SK
Sulfated glycoprotein (prosaposin)	gi\|34328185	3	6	126	ESG	P [23]
Synaptosomal-associated protein 25 (SNAP25)	gi\|26346913	2	10	88	ESG	[62]
Synuclein, beta	gi\|18043841	2	30	103	SK
Thymosin, beta 10	gi\|74199914	2	31	91	SK
Titin isoform N2-A	gi\|77812697	13	1	139	SK
Transferrin receptor 2	gi\|15559221	3	4	52	PM, EN
Triosephosphate isomerase	gi\|54855	3	16	140	Variable
Tropomyosin	gi\|74213492	22	57	959	ESG	[63]
Tubulin, alpha 1a	gi\|74202338	17	50	991	ESG	P [22]
Tubulin, beta	gi\|74141821	21	65	1381	ESG	[64]
Tumor metastatic process-associated protein NM23	gi\|387496	2	17	57	ER, CY
Ubiquitin carboxy-terminal hydrolase L1	gi\|6755929	2	12	60	PM
Ubiquitin protein ligase	gi\|94399459	8	2	85	Variable
Ubiquitin-activating enzyme E1	gi\|74228573	3	4	62	Variable
Vacuolar H+ATPase B2	gi\|74195936	3	5	53	ESG	P [22]
VcpP97 (valosin)	gi\|62738728	4	6	94	ESG	P [22]
Y box transcription factor	gi\|199821	9	36	491	NU