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Novel TSHR Probes. A) The chemical structure of NCGC00161876 (4) and NCGC00166332 (5). B) Molecular model of a potential docking orientation of NCGC00166332 (5) bound to the transmembrane core of TSHR. C) Intracellular cAMP accumulation in HEK-EM 293 cells stably expressing TSHR in the presence of sera from patients with Graves’ disease (GD) or the EC50 concentration of TSH (1.8 nM) in the presence or absence of NCGC00166332 (5).
