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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Insect Mol Biol. 2009 Dec 11;19(2):153–162. doi: 10.1111/j.1365-2583.2009.00965.x

Figure 3. Hierarchical and k-means clustering.

Figure 3

Clustering analysis of the 631 transcripts significantly associated with mating demonstrates that instrumental insemination has a strong effect on brain gene expression. These results match the behavioral patterns that we observed for flight attempts. K-means clustering revealed 4 main clusters of genes. Cluster 1 identifies transcripts that are upregulated by the instrumental insemination process, while Cluster 2 depicts transcripts downregulated by the instrumental insemination process. Clusters 3 and 4 identify transcripts associated with the effects of semen exposure. Cluster 3 seems to contain a set of transcripts that are downregulated by semen exposure in mated queens relative to virgins, and in semen- relative to saline-inseminated queens. Cluster 4 depicts transcripts upregulated by exposure to semen and seminal proteins, with these transcripts upregulated in mated and semen-inseminated queens relative to the other groups. Values are zero-centered transcriptional data in the log2 scale; a value of 1 indicates a 2-fold change in expression levels for a given transcript.