Fig. 2.

Effect of parenteral BSI-401 on the tumor growth and survival of athymic mice bearing orthotopic COLO357FG or L3.6pl/GLT pancreatic tumors. Fifteen mice (n= 5) bearing tumors were randomly assigned to be treated with BSI-401 ip as indicated, or DMSO as control. At the median survival duration of the control group, the tumor growth in the mice of all groups was evaluated on the basis of the bioluminescence emitted by the tumor cells. A, D, Tumor volume following treatment, quantified as the sum of all detected photons within the region of the tumor per second. Means and SE are shown. * P< 0.05 treatment versus control by one-way analysis of variance and Dunnett’s multiple comparison post test. B,E, Image of mice bearing luciferase-expressing tumors. A digital grayscale image of each mouse was acquired, which was followed by the acquisition and overlay of a pseudocolor image representing the spatial distribution of detected photons emerging from active luciferase within the mouse. C, F, Survival curve following treatment. Mice were sacrificed by carbon dioxide inhalation when evidence of advanced bulky disease was present. The day of sacrifice was considered the day of death for survival evaluation.