Osteogenic differentiation of hUCMSCs while being encapsulated in the constructs. (A) DNA content, (B) ALP, measured using a colorimetric p-nitrophenyl phosphate (pNPP) assay and normalized by the DNA. Each value is mean ± sd; n = 5. In each plot, bars with dissimilar letters indicate values that are significantly different (p < 0.05). The control hUCMSCs were cultured in control media. All other constructs were cultured in osteogenic media. ALP increased substantially at 7 days, peaked at 14 days, and slightly decreased at 21 days. Chitosan, polyglactin fibers, and CPC had no adverse effects on ALP, when compared to cells in hydrogel without CPC (p > 0.1).