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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Biomaterials. 2010 Feb 10;31(14):3848–3857. doi: 10.1016/j.biomaterials.2010.01.093

Fig. 4.

Fig. 4

Osteogenic differentiation of hUCMSCs while being encapsulated in the constructs. (A) DNA content, (B) ALP, measured using a colorimetric p-nitrophenyl phosphate (pNPP) assay and normalized by the DNA. Each value is mean ± sd; n = 5. In each plot, bars with dissimilar letters indicate values that are significantly different (p < 0.05). The control hUCMSCs were cultured in control media. All other constructs were cultured in osteogenic media. ALP increased substantially at 7 days, peaked at 14 days, and slightly decreased at 21 days. Chitosan, polyglactin fibers, and CPC had no adverse effects on ALP, when compared to cells in hydrogel without CPC (p > 0.1).