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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Biomaterials. 2010 Feb 10;31(14):3848–3857. doi: 10.1016/j.biomaterials.2010.01.093

Fig. 5.

Fig. 5

hUCMSC mineralization while encapsulated in the constructs. Four constructs were tested: (1) hUCMSCs in hydrogel beads; (2) hUCMSCs in beads in CPC; (3) hUCMSCs in beads in CPC–chitosan; (4) hUCMSCs in beads in CPC-chitosan-fiber scaffold. hUCMSCs osteo-differentiated and synthesized minerals in all four constructs. Mineral content stained by xylenol orange emitted red fluorescence. Mineralization increased with time. Adding chitosan and fibers, which greatly improved the load-bearing properties, did not compromise minerlization by the hUCMSCs in CPC.