Table 2.
Identification of candidate biomarkers obesity and diabetes1.
| Protein Name2 | Protein MW (Da)3 | Protein pI4 |
MS/MS Search Score5 |
Distinct Peptides6 | Spectral Intensity (×107) for respective patients ID7 |
|||
|---|---|---|---|---|---|---|---|---|
| 1-A | 3-B | 5-C | 8-D | |||||
| Apolipoprotein B precursor | 515533.1 | 6.58 | 640.05 | 49 | 5.96 | 16.8 | 17.2 | 4.20 |
| Complement component 4A | 192788.6 | 6.72 | 298.16 | 21 | 22.3 | 25.5 | 21.0 | 4.99 |
| Ceruloplasmin | 123002.7 | 5.46 | 281.99 | 19 | 35.1 | 27.7 | 51.3 | 9.77 |
| Fibronectin 1 | 272337.8 | 5.3 | 210.73 | 16 | 4.83 | 6.28 | 11.9 | 1.71 |
| Inter-alpha-trypsin inhibitor family heavy chain-related protein | 103385.9 | 6.51 | 153.9 | 11 | 40.6 | 33.2 | 29.9 | 4.53 |
| Inter-alpha inhibitor H1 | 101366.7 | 6.43 | 143.71 | 10 | 8.85 | 13.5 | 9.96 | 3.81 |
| Hemopexin | 51676.7 | 6.55 | 140.64 | 9 | 39.3 | 61.8 | 48.2 | 18.6 |
| Apolipoprotein A-1 preproprotein | 30778 | 5.56 | 117.16 | 7 | 3.33 | 39.3 | 27.9 | 11.7 |
| Beta-2-glycoprotein 1 apolipoprotein H | 38312.5 | 8.34 | 101.84 | 7 | 87.9 | 41.2 | 70.6 | 6.05 |
| Alpha-1B-glycoprotein precursor | 54272.8 | 5.58 | 100.81 | 7 | 26.9 | 32.3 | 34.1 | 7.33 |
| Angiotensinogen precursor variant | 53778.2 | 5.97 | 87.81 | 6 | 36.9 | 36.8 | 71.6 | 6.26 |
LC-MS/MS Spectrum Mill database-search for plasma samples obtained from control obese and/or diabetic patients.
List of the protein.
Theoretical MW (Da) of the identified protein from the database.
Theoretical isolelectric points (pI) of the identified protein from the database.
MS/MS search score is computed by scoring function that measure the degree of similarity between the experimental spectrum and the theoretical fragmentation patterns of the candidate peptides.
Number of the distinct peptides of the protein after fragmentation and is based on the homology of the protein with the known protein in the database.
The intensity fold increase of the spectra in the sample is calculated by the factor 1×107.