Figure 3.

Polyreactivity of IgG⁺ antibodies from GC B cells and plasma cells from FcγRIIB^−/− and wild-type mice. (A) Antibodies cloned (black lines) from IgG⁺ GC B cells and spleen and bone marrow plasma cells of FcγRIIB^−/− (1RII– 5RII) and wild-type (1B6–3B6) mice were tested for polyreactivity with dsDNA, insulin, and LPS by ELISA. Graphs show OD₄₀₅ values at 1 µg/ml antibody concentration and three consecutive 1:4 dilutions of representative antibodies. Dashed lines represent the highly polyreactive positive control antibody ED38 (Wardemann et al., 2003). Red lines represent the low-polyreactive positive control antibody JB40 and green lines represent the nonpolyreactive negative control antibody mGO53 (Wardemann et al., 2003). (B) Pie charts summarize the frequency of nonpolyreactive antibodies (white) and polyreactive antibodies (black) in the respective compartments. All antibodies were tested in at least three independent experiments. The numbers of antibodies analyzed are indicated in the pie chart centers. P-values were calculated by Fisher’s exact 2 × 2 test.