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. 2010 Nov 22;207(12):2675–2687. doi: 10.1084/jem.20091573

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© 2010 Ebihara et al.

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Figure 5. — INAM is not an NK-activating ligand. (A) Flow cytometry for Rae-1 and Flag-tagged INAM in stable BaF3 lines. Shaded peak, untransfected control BaF3 staining with anti–pan-Rae-1 Ab or anti-Flag M2 antibody; open peak, stable Rae-1α/BaF3 or stable Flag-tagged INAM/BaF3 staining with anti–pan-Rae-1 antibody or anti-Flag M2 antibody. (B) Cytotoxicity against control BaF3, Rae-1α/BaF3, and INAM/BaF3 by NK cells treated with 1,000 IU/ml IL-2 for 3 d. Data shown are means ± SD of triplicate samples from one experiment representative of three. (C) NK activation is augmented by coexistent BMDC irrespective of INAM expression. NK cells were cultured with 1,000 IU/ml IL-2 for 3 d. 2 × 10⁵ NK cells, 10⁵ BaF3 cells, and 10⁵ IRF-3^−/− BMDCs were co-cultured in 200 µl/well and IFN-γ in the supernatants were measured by ELISA. Data show one of two similar experimental results. Data represent mean ± SD.