Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Nov 22;207(12):2689–2701. doi: 10.1084/jem.20101111

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 Baumann et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 2. — CD14 is an interactor of TLR3, TLR7, TLR8, and TLR9. (A) Myc-tagged CD14 was double transfected in Hek293T cells with either one V5-tagged endosomal TLR or V5–IL-1R as a negative control. 48 h after transfection, V5-tagged proteins were immunoprecipitated out of cell lysates using V5 agarose. Coprecipitation of CD14 was analyzed by Western blotting for myc and V5. IB, immunoblot. (B) Myc-CD14 was cotransfected with V5-TLR3, -TLR7, -TLR8, or -TLR9 into HeLaS3 cells. Colocalization was analyzed by confocal imaging. Representative areas of overlapping localization are shown magnified in the insets in the merge panel. (C) Images of a confocal section of endogenous CD14 and TLR9 in unstimulated RAW264.7 macrophages. (bottom) Quantification of three-dimensional colocalization analysis. Graph shows the percentage of pixels positive for CD14 colocalizing with pixels positive for TLR9 and the percentage of pixels positive for TLR9 colocalizing with pixels positive for CD14. (D) Colocalization analysis of the endosomal marker protein Eea1 and CD14 in unstimulated RAW264.7 macrophages. Data in A–D are representative of three independent experiments. Quantitation in C is a mean of two independent experiments with each n = 600. Bars: (B) 10 µm; (C and D) 5 µm.