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. 2010 Jun 15;25(12):3890–3897. doi: 10.1093/ndt/gfq325

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© The Author 2010. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

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Fig. 4 — C1β3Gal-T and Cosmc mRNA expression in response to cytokine stimulation. The level of mRNA encoding either C1β3Gal-T (A) or Cosmc (B) after addition of IL-4 was significantly less than that of control cells at each incubation time (P < 0.05 and P < 0.005, respectively). No significant difference was found between IFN-γ and control cultures at any incubation time. C1β3Gal-T/Cosmc ratio of mRNA expression with IL-4 stimulation was higher at 12 h than that of other incubation time (C). The effect of IL-4 on Cosmc mRNA levels was significantly greater than that on C1β3Gal-T at 12 and 24 h (P < 0.05). The efficiencies and the correlation index that were calculated from the slopes of the standard curves of C1β3Gal-T, Cosmc and β-actin were 0.984931–1.130224 and 0.98917–0.991649, respectively. Results are expressed as a relative ratio to control (A and B) or a ratio of C1β3Gal-T/Cosmc (C) of each incubation time, and mean ± SE.