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. Author manuscript; available in PMC: 2010 Nov 22.
Published in final edited form as: Cell Cycle. 2008 Nov 9;7(21):3320–3326. doi: 10.4161/cc.7.21.6969

Figure 5.

Figure 5

Effect of delphinidin on NFκB/p65 transcriptional activation and the protein levels of pIKKγ, pIκBα, total IκBα in 22Rν1 cells. (A) Effect of delphinidin treatment on NFκB promoter activity. Cells were transiently co-transfected with NFκB luciferase plasmid and Renilla luciferase plasmids for 12 hours and cells were treated with vehicle only or specified concentrations of delphinidin for 24 hours and harvested as detailed in Materials and Methods. (B) Protein levels of pIKKγ, pIκBα and total IκBα in cells treated with vehicle alone (0.1% DMSO) alone and specified concentrations of delphinidin in (0.1% DMSO) as determined by Western blot analysis. Equal loading of protein was confirmed by stripping and reprobing the blots with β-actin antibody.