Table II.
Overrepresented canonical pathways in comparisons among ICSIKSOM, ICSIWM and in vivo embryos (P < 0.05).
| Ingenuity canonical pathways | Ratio | Gene symbol |
|---|---|---|
| Altered pathways with fold change in ICSI embryos (both ICSIWM and ICSIKSOM) compared with in vivo embryos | ||
| Signaling | ||
| GABA receptor signaling | 0.109 | Abat (4.6, 3.6), Gabra5 ( −3.1, −3.3), Gabrb2 (−2.7, −2.5), Gabrg1 (−2.2, −2.3), Slc6a13 (6.5, 5.7) |
| Invasiveness signaling (Glioma) | 0.107 | F2r (3.6, 5.3), Itgb5 (4.2, 4.5), Rhod (5.3, 4.4), Rhoj (−2.7, −2.7), Rras (3.7, 3.9), Timp1 (4.8, 5.9) |
| Metabolism | ||
| Inositol metabolism | 0.143 | Adi1 (−2.5, −2.5), Aldh16a1 (3.3, 3.7), Blvrb (9.5, 11.1), Cyba (6.5, 8.5), Dhrs1 (5.4, 4.3), Dhrs13 (3.7, 3.7), Lamb2 (4.7, 4.5), Ndor1 (4.1, 4.1), Ndufa6 (3.6, 3.4), Recql4 (3.7, 3.5), Retsat (3.3, 3.5), Tm7sf2 (3.9, 3.6) |
| Butanoate metabolism | 0.133 | Abat (4.6, 3.6), Aldh2 (3.5, 3.5), Dcxr (14.3, 15.1), Ech1 (4.4, 5.7), Hadha (4.0, 4.1), Nlgn1 (−2.6, −2.8), Oxct1 (3.9, 4.1), Sdhb (3.8, 4.0) |
| N-glycan degradation | 0.133 | Man1b1 (4.1, 3.4), Man1c1 (3.1, 4.2), Man2c1 (3.8, 4.2), Manea (3.2, 3.5) |
| Urea cycle and metabolism of amino groups | 0.118 | Acy3 (6.1, 4.9), Ass1 (4.1, 3.7), Oat (3.4, 4.0), Pycrl (4.0, 3.4) |
| Valine, leucine and isoleucine degradation | 0.101 | Abat (4.6, 3.6), Aldh2 (3.5, 3.5), Bcat2 (4.7, 5.4), Bckdhb (4.4, 4.5), Ech1 (4.4, 5.7), Hadha (4.0, 4.1), Oxct1 (3.9, 4.1) |
| Glutathione metabolism | 0.094 | Gpx3 (8.0, 7.7), Gpx7 (5.3, 6.2), Gstk1 (5.8, 4.7), Gstm2 (10.6, 13.4), Mgst3 (4.3, 3.9), Oplah (3.9, 4.0) |
| Mitochondrial function | ||
| Ubiquinone biosynthesis | 0.127 | Bckdhb (4.4, 4.5), Ndufa1 (4.0, 3.9), Ndufa13 (3.8, 3.6), Ndufa3 (4.1, 4.2), Ndufa6 (3.6, 3.4), Ndufb3 (3.8, 4.0), Ndufb7 (4.1, 4.0), Ndufs7 (4.4, 4.2), Ndufs8 (3.3, 3.3) |
| Oxidative phosphorylation | 0.105 | Atp5e (3.4, 3.7), Atp5g2 (3.2, 3.9), Cox6b2 (22.9, 24.9), Cox7a1 (7.0, 5.8), Ndufa1 (4.0, 3.9), Ndufa13 (3.8, 3.6), Ndufa3 (4.1, 4.2), Ndufa6 (3.6, 3.4), Ndufb3 (3.8, 4.0), Ndufb7 (4.1, 4.0), Ndufs7 (4.4, 4.2), Ndufs8 (3.3, 3.3), Ppa2 (6.6, 7.7), Sdhb (3.8, 4.0), Uqcr (4.1, 4.2) |
| Mitochondrial dysfunction | 0.092 | Cox6b2 (22.9, 24.9), Cox7a1 (7.0, 5.8), Gpx7 (5.3, 6.2), Ndufa13 (3.8, 3.6), Ndufa3 (4.1, 4.2), Ndufa6 (3.6, 3.4), Ndufb3 (3.8, 4.0), Ndufb7 (4.1, 4.0), Ndufs7 (4.4, 4.2), Ndufs8 (3.3, 3.3), Sdhb (3.8, 4.0), Snca (−2.6, −2.6) |
| Pathways uniquely changed between ICSIWM and in vivo embryos | ||
| Signaling | ||
| Role of Oct4 in mammalian embryonic stem cell pluripotency | 0.044 | Fgf4 (−2.3), Rxrb (3.5) |
| CDK5 signaling | 0.034 | Egr1 (−2.6), Ppp1r11 (3.3), Ppp1r3a (−2.5) |
| TR/RXR activation | 0.033 | Ppargc1a (−2.2), Rxrb (3.5), Ucp2 (3.2) |
| Production of nitric oxide and reactive oxygen species in macrophages | 0.031 | Map3k4 (3.7), Mapk13 (3.9), Ppp1r11 (3.3), Ppp1r3a (−2.5), Rnd2 (3.8) |
| Pathways uniquely changed between ICSIKSOM and in vivo embryos | ||
| Signaling | ||
| HGF signaling | 0.04 | Ccnd1 (−2.2), Elf3 (3.4), Elf5 (3.7), Map3k6 (3.5) |
| Metabolism | ||
| Glycolysis/gluconeogenesis | 0.042 | Aldh3b2 (3.4), Galm (3.2), Ldha (4.0), Pfkm (3.7) |
| Pathways uniquely changed between ICSIKSOM and ICSIWM embryos | ||
| Signaling | ||
| RhoA signaling pathways | 0.019 | Myl9 (2.05), Sept4 (2.04) |
| Metabolism | ||
| One-carbon pool by folate | 0.048 | Mthfd2 (2.33) |
| Pathways changed between IVFWM and ICSIWM | ||
| Signaling | ||
| Invasiveness signaling (Glioma) | 0.125 | F2r (−7.87), Itgb5 (−3.41), Rhod (−3.28), Rhoj (2.35), Rnd2 (−2.86), Rras (−4.83), Timp1 (−6.65) |
| Metabolism | ||
| Urea cycle and metabolism of amino groups | 0.176 | Acy3 (−3.30), Aldh18a1 (−5.21), Cps1 (3.72), Oat (−3.77), Pycrl (−3.31), Srm (−4.52) |
| Glutathione metabolism | 0.125 | Ggt1 (−3.80), Gpx4 (−3.72), Gpx7 (−4.64), Gstk1 (−3.40), Gstm2 (−5.77), Mgst1 (2.19), Mgst3 (−3.05), Oplah (−5.19) |
| Phospholipid degradation | 0.103 | Dgka (−3.23), Hmox1 (−3.99), Lamb2 (−3.89), Napepld (−3.84), Pla1a (−3.25), Pla2g10 (−2.96), Plcd1 (−5.23), Pld3 (−3.05), Sphk2 (−3.39) |
| Selenoamino acid metabolism | 0.132 | Cbs (−5.85), Cth (−5.93), Edf1 (−3.16), Ggt1 (−3.80), Mars2 (−3.00) |
| Glycerophospholipid metabolism | 0.092 | Agpat2 (−3.34), Dgka (−3.23), Hmox1 (−3.99), Lamb2 (−3.89), Napepld (−3.84), Pcyt2 (−4.84), Pemt (−2.59), Pla1a (−3.25), Pla2g10 (−2.96), Plcd1 (−5.23), Pld3 (−3.05), Sphk2 (−3.39) |
| Glutamate metabolism | 0.132 | Abat (−5.49), Cad (−4.01), Cps1 (3.72), Nadsyn1 (−2.47), Tgm4 (−3.55) |
| Inositol metabolism | 0.131 | Adi1 (2.20), Aldh16a1 (−3.80), Blvrb (−6.23), Cyba (−7.99), Dhrs1 (−4.05), Dhrs13 (−3.73), Hmox1 (−3.99), Lamb2 (−3.89), Ndor1 (−3.62), Recql4 (−4.66), Tm7sf2 (−4.01) |
| Pyrimidine metabolism | 0.111 | Apobec1 (2.38), Cad (−4.01), Dhodh (−4.03), Mad2l2 (−3.24), Nme3 (−5.20), Nme4 (−3.70), Nt5m (−3.21), Pold1 (−4.68), Pold2 (−9.11), Pold4 (−3.58), Poli (−3.79), Polr2i (−5.06), Polr2l (−5.06), Polrmt (−3.35), Trub2 (−3.57), Uckl1 (−3.20), Upp1 (−3.39) |
| Mitochondrial function | ||
| Mitochondrial dysfunction | 0.115 | Cox6b2 (−14.50), Cox7a1 (−5.05), Cox7b2 (−2.33), Dhodh (−4.03), Gpx4 (−3.72), Gpx7 (−4.64), Hsd17b10 (−3.24), Ndufa3 (−4.04), Ndufb3 (−3.61), Ndufb7 (−3.25), Ndufs2 (−3.71), Ndufs7 (−3.88), Ndufs8 (−3.32), Park7 (−3.28), Ucp2 (−3.22) |
| Oxidative phosphorylation | 0.091 | Atp5e (−3.69), Cox6b2 (−14.50), Cox7a1 (−5.05), Cox7b2 (−2.33), Ndufa1 (−3.10), Ndufa3 (−4.04), Ndufb3 (−3.61), Ndufb7 (−3.25), Ndufs2 (−3.71), Ndufs7 (−3.88), Ndufs8 (−3.32), Ppa2 (−5.10), Uqcr (−4.16) |
| Ubiquinone biosynthesis | 0.127 | Bckdhb (−4.41), Edf1 (−3.16), Ndufa1 (−3.10), Ndufa3 (−4.04), Ndufb3 (−3.61), Ndufb7 (−3.25), Ndufs2 (−3.71), Ndufs7 (−3.88), Ndufs8 (−3.32) |