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. Author manuscript; available in PMC: 2010 Nov 22.
Published in final edited form as: J Immunol. 2009 Apr 15;182(8):4616–4623. doi: 10.4049/jimmunol.0801279

FIGURE 2.

FIGURE 2

IL-10 is an important mediator in the regulation of BMDC by NECA. A, BMDC were treated in presence or absence of LPS and 250 nM NECA. At various time points, cells were lysed, RNA was isolated, and real-time RT-PCR was conducted to determine IL-10 mRNA levels. Transcripts were normalized to the 1-h media control treatment group. Data represent the mean ± SEM from three or more experiments. B, Representative histogram of CD86 expression 24 h after BMDC treated in the presence or absence of LPS, 250 nM NECA, or 10 μg/ml anti-IL-10 neutralizing Ab. C, BMDC were treated with 1 μM NECA or DMSO vehicle control in the presence or absence of 500 ng/ml LPS, and after 18 h supernatant was collected. Anti-IL-10 neutralizing Ab (10 μg/ml) was added where indicated. TNF-α and IL-12p70 were assayed by ELISA. Data represent the mean ± SEM from two experiments conducted in duplicate. *, p < 0.05.