Figure 2.

Low levels of WT hSOD1 aggregates in spinal cords of older Gurney mice. Spinal cords from Gurney strains (SJL and Cg) of mice, but not from L76 mice, contain detergent-insoluble aggregates of WT SOD1. (A) Immunoblots of S1 and P2 fractions of mice expressing WT hSOD1 at varied ages. An antibody that recognizes mouse and hSOD1 was used. NTg, non-transgenic mice. Gn.G37R denotes symptomatic aged mice (∼7 months) that harbor human genomic fragments of the SOD1 gene (line 29)—mice that were originally described by Wong et al. (16). (B) Quantification of the aggregation index. Unpaired Student's t-tests were performed to establish significant differences as indicated: *P ≤ 0.05, ^#P ≤ 0.005. Bars represent mean ± SEM.