Figure 1. Ynd1 constructs.

A schematic representation of WT and mutant Ynd1 proteins is shown. The dark grey lines in the WT protein represent apyrase-conserved-domains found in the N-terminal, luminal part of the protein, and the light grey lines represent hydrophobic domains. The rightmost hydrophobic domain was determined to be the transmembrane domain [26]. All Ynd1 constructs carry a Myc tag at their amino terminus (N) and a HA tag at the carboxyl terminus (C).