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Expected and observed codon compositions. We chose four codons randomly as examples: AAT (A to C), TGC (D to F), GCC (G to I), and CTT (J to L). The expected and observed codon compositions were quantitated in Archaea (A, D, G and J), Bacteria (B, E, H and K), and Eukarya (C, F, I and L). Each point in all plots represents a sum of the composition from the species coding sequences and similar plots for all other codons were summarized in Additional file 3.
