Table 3.
Features of PCR-amplified mycobacterial rhomboids
| Primer | Species/Strain | Amplicon size (bp) | ORF (bp) | Amino acids | Accession numberh | Protein ID | |
|---|---|---|---|---|---|---|---|
| Orthologs of Rv0110 (rhomboid protease 1) | 0110F 0110R |
aH37Rv | 967 | 855 | 284 | HM453890 | ADO17908 |
| bBCG | 967 | 855 | 284 | HM453894 | ADO17912 | ||
| cJN55 | 967 | 855 | 284 | HM453896 | ADO17914 | ||
| dBN44 | 967 | 855 | 284 | HM453892 | ADO17910 | ||
| 5036F 5036R |
eSMR5 | 1000 | 891 | 296 | HM453900 | ADO17919 | |
| Orthologs of Rv1337 (rhomboid protease 2) | 1337F 1337R |
H37Rv | 869 | 723 | 240 | HM453891 | ADO17909 |
| BCG | 869 | 723 | 240 | HM453895 | ADO17913 | ||
| JN55 | 869 | 723 | 240 | HM453897 | ADO17915 | ||
| BN44 | 869 | 723 | 240 | HM453897 | ADO17911 | ||
| 1554F 1554R |
fSU-36800 | 954 | 672 | 223 | HM453898 | ADO17916 | |
| g27 | 954 | 291 (MAP2426c) | 72 | HM453899 | ADO17917 | ||
| 444 (MAP2425c) | 147 | HM453899 | ADO17917 | ||||
| 4904F 4904R |
SMR5 | 845 | 738 | 245 | HM453901 | ADO17920 | |
a: M. tuberculosis
b: M. bovis
c: M. bovis (cattle isolate)
d: M. tuberculosis (patient isolate)
e: M. smegmatis (streptomycin resistant derivative of MC2155)
f: M. avium (patient isolate)
g: M. avium subsp. Paratuberculosis (cattle isolate)
h: Accession numbers are for GenBank
Primer sequences are described in methods