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. Author manuscript; available in PMC: 2011 Dec 21.
Published in final edited form as: Mol Microbiol. 2010 Jun 21;77(4):972–994. doi: 10.1111/j.1365-2958.2010.07263.x

Figure 5. Influence of nitrogen availability and ambient pH on gene expression in a Ffvel1 knock-out strain compared to the F. fujikuroi wild type IMI58289.

Figure 5

A Expression of bik1-3, cps/ks, ggs2 and Ffvel1 in the wild type, the Ffvel1 and areA knockout mutants. The strains were grown for 72 h in 10 % ICI medium, washed and shifted into 0 % ICI or 100 % ICI medium, respectively, for 2 h. 28S and 18S rRNA was visualized by EtBr staining as control.

B Expression of bik2, cps/ks, ggs2, areA, areB, nmrA and pacC in the wild type and the Ffvel1 knock-out mutant. The strains were grown for 72 h in 10 % ICI or 100 % ICI medium containing glutamine or NaNO3, respectively. 28S and 18S rRNA was visualized by EtBr staining as control.

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