Figure 9. Phenotypical and secondary metabolite analysis in the Ffvel2 knock-out strain compared to the F. fujikuroi wild type IMI58289.

A Photographs of the F. fujikuroi wild type strain IMI58289, the Ffvel1 and Ffvel2 knockout strain and the overexpression strain OE::Ffvel1 grown on different solidified media for 10 days under constant dark conditions. V8 = vegetable juice; CM = complete medium; PDA = Potatoe Dextrose Agar; CD = Czapek Dox (for details see Experimental Procedures).

B Spores produced by the F. fujikuroi wild type strain IMI58289, the Ffvel1 and Ffvel2 knock-out strains and the overexpression strain OE::Ffvel1 grown for 10 days on V8 solidified media under constant light conditions. Experiment was carried out in triplicate; bars show standard deviations.

C Expression of selected gibberellin and bikaverin genes in the wild type and the Ffvel1 and Ffvel2 knock-out strains. The strains were grown for the indicated time in 10 % ICI medium. The northern blot was hybridized with the indicated probes. 28S and 18S rRNA was visualized by EtBr staining as control.

D Photographs of the F. fujikuroi wild type strain IMI58289, the Ffvel1 and Ffvel2 knock-out strains grown in 10 % ICI cultures for 3 days.

E Thin layer chromatogram of the F. fujikuroi wild type strain IMI58289 and the Ffvel1 and Ffvel2 knock-out strains grown in 10 % ICI cultures for the indicated time. GA₃ was used as standard (for details see Experimental procedures).