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Influence of different protocol variations. Comparison of average protein identifications after a protein extraction with urea lysis buffer or 0.1% TFA, b digestion with the in-solution or the in-gel approach, c digestion with trypsin or AspN, d MS/MS fragmentation and detection by IT-CID or IT-ETcaD, and e MS/MS database search using the MASCOT or SEQUEST search engines
