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Detection of the recombinant proteins rKMPII, rpapLe22, rTRYP, and rLACK in T. ni larvae. Total protein extracts were analyzed by (A) Coomassie brilliant blue staining of SDS-PAGE gels and (B) Western blotting using a pool of canine sera from total Leishmania antigen-positive samples. Arrows indicate the position of the recombinant proteins.
